| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EDS4A; EDSVASC |
| Entrez GeneID | 1281 |
| WB Predicted band size | Calculated MW: 139 kDa; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human Collagen III |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **"Type III collagen is crucial for collagen fibrillogenesis and viability in the developing mouse"**
- Authors: Li, S.W., et al.
- Summary: 研究通过基因敲除小鼠模型，证实COL3A1在胶原纤维形成中的关键作用，并利用特异性抗体检测组织中的III型胶原分布，揭示其在胚胎发育中的必要性。
2. **"Antibody-based validation of extracellular matrix in chronic fibrosis"**
- Authors: Humphreys, B.D., et al.
- Summary: 评估多种细胞外基质蛋白抗体(包括COL3A1抗体)在纤维化组织中的特异性，通过免疫组化和Western blot验证其在肾脏、肝脏纤维化模型中的表达上调。
3. **"Collagen III deposition is a driver of cardiac fibrosis via integrin signaling"**
- Authors: Leask, A., et al.
- Summary: 利用COL3A1抗体研究心脏纤维化机制，发现III型胶原通过整合素信号通路促进成纤维细胞活化，抗体染色显示其在心肌梗死模型中的异常沉积。
4. **"Proteomic analysis identifies collagen III as a biomarker of severe pulmonary hypertension"**
- Authors: Rhodes, C.J., et al.
- Summary: 通过质谱和免疫荧光技术，发现肺高压患者肺组织中COL3A1显著升高，其抗体被用于定量分析，提示III型胶原可作为疾病严重程度的生物标志物。
以上文献涵盖抗体在发育生物学、纤维化机制、心血管疾病及临床诊断中的应用，强调其作为研究工具的价值。
The Collagen III alpha 1 (COL3A1) antibody is a critical tool for studying the COL3A1 gene product, a key component of type III collagen, which belongs to the fibrillar collagen family. Type III collagen, a homotrimer of three alpha-1(III) chains, is predominantly found in extensible tissues such as skin, blood vessels, and internal organs, where it provides structural integrity and elasticity. It is secreted by fibroblasts and other mesenchymal cells, playing essential roles in extracellular matrix (ECM) assembly, tissue repair, and embryonic development. COL3A1 mutations are linked to vascular Ehlers-Danlos syndrome (vEDS), a rare genetic disorder characterized by fragile blood vessels and organ rupture.
Antibodies targeting COL3A1 are widely used in research to investigate fibrotic diseases (e.g., liver cirrhosis, pulmonary fibrosis), cardiovascular disorders, and cancer, where abnormal collagen III deposition or turnover is implicated. These antibodies enable detection of COL3A1 expression via techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence, aiding in the characterization of ECM remodeling in pathological conditions. Monoclonal and polyclonal variants are available, with species cross-reactivity typically validated for human, mouse, and rat samples.
Understanding COL3A1 dynamics through antibody-based assays contributes to insights into tissue fibrosis progression, wound healing mechanisms, and potential therapeutic targets for ECM-related diseases. Its diagnostic utility in confirming vEDS and research applications in regenerative medicine further underscore its importance.
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