首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胰蛋白酶3(PRSS3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PRSS3含量。PRSS3是颇具研究价值的靶点。它在生理与病理过程中发挥作用，与多种疾病发展相关。研究机制上，主要聚焦于其在疾病信号通路里的具体功能，以及与其他生物分子的相互作用，以探寻通过调控它来治疗疾病的可能性。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本产品适用于体外实验环境下探索PRSS3在消化系统疾病、炎症反应或肿瘤微环境中的分子机制研究，其高灵敏度和宽线性范围可满足基础科研中对细胞培养上清、动物模型血清或组织提取物的检测需求，为研究PRSS3的表达调控、功能验证及其作为潜在生物标志物的探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Brain trypsinogen ELISA Kit; Mesotrypsin ELISA Kit; Mesotrypsinogen ELISA Kit; MTG ELISA Kit; Pancreatic trypsinogen III ELISA Kit; Protease, serine, 3 ELISA Kit; Protease, serine, 4 (trypsin 4, brain) ELISA Kit; PRSS3 ELISA Kit; PRSS4 ELISA Kit; Serine protease 3 ELISA Kit; Serine protease 4 ELISA Kit; T9 ELISA Kit; TRY3 ELISA Kit; TRY3_HUMAN ELISA Kit; TRY4 ELISA Kit; Trypsin 3 ELISA Kit; Trypsin III ELISA Kit; Trypsin IV ELISA Kit; Trypsin-3 ELISA Kit; Trypsinogen 4 ELISA Kit; Trypsinogen 5 ELISA Kit; Trypsinogen IV ELISA Kit
缩写：PRSS3
Uniprot No.：P35030
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 92 | |||||
| Range % | 87-96 | ||||||
| 1:200 | Average % | 103 | |||||
| Range % | 98-107 | ||||||
| 1:400 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
| 1:800 | Average % | 92 | |||||
| Range % | 86-98 | ||||||
回收率：
| The recovery of human PRSS3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 86-98 | |||||
| EDTA plasma (n=4) | 97 | 91-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.5842.6872.6362.5525002.2952.1652.2302.1462501.5941.5231.5591.4751250.8890.8760.8830.79962.50.4410.4540.4480.36431.20.2610.2520.2570.17315.60.1430.1480.1460.06200.0830.0840.084 | |||||||
货期：3-5 working days
×
