首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：犬补体蛋白4(C4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C4含量。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，适用于动物模型研究、免疫系统功能评估、药物干预效果观测等科研场景，为犬类基础医学研究提供标准化工具；其浓度变化可为研究犬类免疫相关疾病机制、感染性疾病进程或自身免疫异常提供重要依据本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C4
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.8 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:400 | Average % | 96 | ||||
| Range % | 92-99 | |||||
| 1:800 | Average % | 103 | ||||
| Range % | 98-109 | |||||
| 1:1600 | Average % | 87 | ||||
| Range % | 82-91 | |||||
回收率：
| The recovery of dog C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 90 | 87-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average20000.0800.0790.08010000.1170.1250.1215000.2020.1980.2002500.3110.3050.3081250.4990.5130.50662.50.9270.9330.93031.251.4571.3421.40002.5592.4382.499 | ||||||
货期：3-5 working days
×
