首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人多配体聚糖4(SDC4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的SDC4含量。SDC4（Syndecan-4）是一种位于人类染色体20q13.12上的蛋白质编码基因，编码的蛋白在肝、肺等组织中广泛表达。SDC4与细胞骨架蛋白结合和纤连蛋白结合相关，参与细胞外基质降解和信号传导。其研究机制涉及细胞与细胞外基质相互作用，在血管损伤后新生内膜形成、肿瘤侵袭等过程中发挥重要作用。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于探究SDC4在肿瘤生物学行为、炎症微环境调控或细胞基质相互作用中的分子机制，也可用于疾病模型构建过程中生物标志物的动态监测，为细胞信号传导、组织修复等基础研究领域提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：SDC4
Uniprot No.：P31431
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SDC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 90-96 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human SDC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 94 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.8592.7832.8212.647202.0372.0052.0211.847101.2451.3041.2751.10150.7650.7820.7740.6002.50.5010.5110.5060.3321.250.3490.3530.3510.1770.6250.2610.2570.2590.08500.1750.1720.174 | ||||||
货期：3-5 working days
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