Bovine Trypsin ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL018811BO

Price： ￥3000

48T 96T

数量：

大包装询价

产品描述：
牛胰蛋白酶酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Trypsin含量。Trypsin即胰蛋白酶，是一种在消化过程中起关键作用的丝氨酸蛋白酶，主要由胰腺分泌。其研究机制主要聚焦在它对肽键的水解作用，参与蛋白质的消化。此外在细胞培养、疾病诊断和治疗方面也有研究应用。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，主要应用于畜牧科研中反刍动物消化生理研究、胰酶代谢调控机制探索，或生物制药领域酶制剂活性检测等场景，为蛋白质代谢相关的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
Trypsin
种属：
Bos taurus (Bovine)
样本类型：
serum, plasma, tissue homogenates
检测范围：
62.5 pg/mL-4000 pg/mL
灵敏度：
15.6 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-96
1:200	Average %	98
	Range %	91-105
1:400	Average %	87
	Range %	82-94
1:800	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average
4000	0.180	0.174	0.177
2000	0.275	0.267	0.271
1000	0.429	0.420	0.424
500	0.613	0.629	0.621
250	0.985	1.021	1.003
125	1.581	1.653	1.617
62.5	2.093	2.184	2.139
0	2.652	2.725	2.688