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产品描述：仓鼠白介素4(IL4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL4含量。IL4是重要的免疫细胞因子靶点。其背景在于它在免疫调节中扮演关键角色，参与Th2细胞分化等过程。研究机制上，主要聚焦其与受体结合后的信号传导通路，以及在过敏、哮喘等疾病发生发展中的作用，旨在开发相关靶向疗法。试剂盒检测范围为9.37 pg/mL-600 pg/mL，适用于仓鼠免疫机制研究、炎症性疾病模型评估、疫苗或药物开发中的免疫应答分析等科研场景，为探究Th2型免疫反应、过敏性疾病机制或感染病理过程提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写：IL4
Uniprot No.：Q60440
种属：Mesocricetus auratus (Golden hamster)
样本类型：serum, plasma, tissue homogenates
检测范围：9.37 pg/mL-600 pg/mL
灵敏度：2.34 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 90-101 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 100 | |||||
| Range % | 98-104 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 87-97 | ||||||
回收率：
| The recovery of hamster IL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 93-99 | |||||
| EDTA plasma (n=4) | 91 | 87-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected6001.8201.8641.8421.6283001.3571.2891.3231.1091500.9300.9630.9470.733750.7060.7390.7230.50937.50.5030.5240.5140.30018.750.3920.3840.3880.1749.370.3040.2880.2960.08200.2160.2120.214 | |||||||
货期：3-5 working days
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