首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人opiorphin酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、唾液样本中的opiorphin含量。opiorphin是一种内源性阿片肽增强剂。其背景在于它是人体天然存在的物质。研究机制方面，它能抑制阿片肽的降解，增强阿片肽活性，从而发挥镇痛、抗焦虑等生理作用，在疼痛治疗和神经精神疾病领域具有潜在研究价值。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于基础科研中镇痛机制探索、精神疾病模型构建及神经肽动态监测等方向。助力科研人员深入探究 Opiorphin 在生理病理过程中的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：opiorphin
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, saliva
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human opiorphin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of human opiorphin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 86 | 81-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.5612.6692.6152.491202.2052.1142.1602.036101.4571.3541.4061.28250.7420.7200.7310.6072.50.4250.4050.4150.2911.250.2750.2830.2790.1550.6250.1870.1810.1840.06000.1230.1240.124 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human opiorphin antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled opiorphin antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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