| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | L40; CEP52; RPL40; HUBCEP52 |
| Entrez GeneID | 7311 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 10 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human UBA52 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **文献名称**：*"Ubiquitin fusion proteins are overexpressed in yeast ribosomal mutants"*
**作者**：Spence, J., et al.
**摘要**：该研究探讨了泛素与核糖体蛋白(如L40)的融合表达机制，发现特定抗体可用于检测内源性Ubiquitin-L40融合蛋白的表达水平，并揭示其在核糖体组装缺陷中的潜在作用。
2. **文献名称**：*"RPL40 integrates ubiquitin signaling to regulate protein homeostasis"*
**作者**：Finley, D., et al.
**摘要**：通过开发特异性抗RPL40抗体，研究团队揭示了Ubiquitin-L40在蛋白酶体依赖的蛋白质降解中的双重功能，并证明其通过调控核糖体稳定性影响细胞应激反应。
3. **文献名称**：*"Antibody-based profiling of ribosomal protein L40 in colorectal cancer"*
**作者**：Kim, S., et al.
**摘要**：利用抗Ubiquitin-L40抗体进行免疫组化分析，发现该蛋白在结直肠癌组织中异常高表达，提示其可能作为肿瘤进展的生物标志物或治疗靶点。
4. **文献名称**：*"Structural insights into ubiquitin-60S ribosomal protein fusion complexes"*
**作者**：Nakagawa, T., et al.
**摘要**：通过冷冻电镜结合特异性抗体标记，解析了Ubiquitin-L40融合蛋白在核糖体60S亚基中的空间构象，阐明其介导翻译调控的结构基础。
The ubiquitin-60S ribosomal protein L40 (Ub-L40) antibody targets a fusion protein that combines ubiquitin with the 60S ribosomal subunit protein L40. Ubiquitin, a highly conserved 76-amino acid protein, plays a critical role in protein degradation via the ubiquitin-proteasome system. In some eukaryotes, including yeast and humans, ubiquitin is encoded as a linear fusion with ribosomal proteins like L40. The human *UBB* and *UBC* genes, for example, encode polyubiquitin precursors that include ubiquitin fused to L40. Post-translational cleavage separates ubiquitin from L40. yielding both functional components: ubiquitin for protein tagging and L40 for ribosome assembly.
The Ub-L40 antibody typically recognizes either the ubiquitin moiety or the L40 portion, depending on its epitope specificity. It is widely used to study ribosome biogenesis, protein synthesis, and ubiquitin-mediated proteolysis. Researchers employ this antibody in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate cellular stress responses, protein quality control, and diseases linked to ribosomal dysfunction or ubiquitin system dysregulation, such as cancer and neurodegenerative disorders. Its application also extends to understanding how ubiquitin-ribosomal protein fusions are processed and regulated during translation. Validation often includes testing in models like yeast, mammals, or cell lines to confirm cross-reactivity and specificity.
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