首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素36α(IL1F6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Il36a含量。Il36a是一个靶点。它在免疫系统中可能发挥一定作用。相关研究机制可能聚焦于其在免疫信号传导通路里的功能，探索它与炎症、疾病发生发展的关联，以明确其在免疫调节中的具体角色，为开发针对性治疗方法提供依据。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可支持科研人员在炎症机制研究、药物干预效果评估或基因编辑模型验证中定量分析IL - 36α的动态变化，尤其适用于免疫代谢研究、感染性疾病模型或组织微环境分析等实验场景，为探索IL - 36α在先天免疫与适应性免疫中的调控网络提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il36a ELISA Kit; Fil1e ELISA Kit; Il1e ELISA Kit; Il1f6 ELISA Kit; Il1h1Interleukin-36 alpha ELISA Kit; FIL1 epsilon ELISA Kit; Interleukin-1 epsilon ELISA Kit; IL-1 epsilon ELISA Kit; Interleukin-1 family member 6 ELISA Kit; IL-1F6 ELISA Kit; Interleukin-1 homolog 1 ELISA Kit; IL-1H1 ELISA Kit
缩写：IL1F6
Uniprot No.：Q9JLA2
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL1F6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-90 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 85-99 | |||||
回收率：
| The recovery of mouse IL1F6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-107 | ||||
| EDTA plasma (n=4) | 88 | 80-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.5912.5872.5892.486 5002.0172.0462.0321.929 2501.3651.3891.3771.274 1250.7990.7510.7750.672 62.50.4550.4830.4690.366 31.20.2610.2430.2520.149 15.60.1690.1860.1780.075 00.1020.1040.103 | ||||||
货期：3-5 working days
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