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产品描述：牛降钙素原(PCT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PCT含量。降钙素原（PCT）是一种蛋白质。在健康人血液中含量极低，而在严重细菌、真菌、寄生虫感染及脓毒症和多脏器功能衰竭时，其水平会异常升高。它可作为炎症和感染的生物标志物，研究其机制有助于深入了解感染进程并指导治疗。试剂盒检测范围为39.06 pg/mL-2500 pg/mL，可为兽医病理研究、畜牧动物健康监测及牛源性疾病模型建立提供关键生物标志物检测支持，特别适用于评估反刍动物炎症反应进程、疫苗效力测试及抗生素治疗研究等基础科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PCT
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue Homogenates
检测范围：39.06 pg/mL-2500 pg/mL
灵敏度：9.77 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 90-97 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 96-97 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of bovine PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-97 | ||||
| EDTA plasma (n=4) | 92 | 90-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 25002.1372.1212.1292.026 12501.5531.5241.5391.436 6251.0331.0071.0200.917 312.50.7050.6870.6960.593 156.250.4360.4120.4240.321 78.120.2820.2730.2780.175 39.060.1800.1670.1740.071 00.1050.1010.103 | ||||||
货期：3-5 working days
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