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产品描述：鱼胰蛋白酶(trypsin)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PRSS1含量。PRSS1是一种编码阳离子胰蛋白酶原的基因，其突变与遗传性胰腺炎相关。该基因突变导致胰蛋白酶原激活增加或失活减少，引发胰腺炎症。研究显示，PRSS1突变是慢性胰腺炎的重要遗传因素，其致病机制涉及胰蛋白酶的异常激活和胰腺组织的损伤。试剂盒检测范围为0.312 ng/mL-20 ng/mL，可为水产动物消化生理研究、疾病模型构建及营养代谢评估提供重要指标；支持实验室开展鱼类消化系统功能研究、水产养殖基础科研以及环境因子对鱼类生理影响的毒理学评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Trypsin-1 ELISA kit; EC 3.4.21.4 ELISA kit; Trypsin I ELISA kit
缩写：PRSS1
Uniprot No.：P35031
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 101 | |||||
| Range % | 95-105 | ||||||
| 1:40 | Average % | 91 | |||||
| Range % | 84-95 | ||||||
| 1:80 | Average % | 106 | |||||
| Range % | 100-111 | ||||||
| 1:160 | Average % | 104 | |||||
| Range % | 97-108 | ||||||
回收率：
| The recovery of fish trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 87-100 | |||||
| EDTA plasma (n=4) | 102 | 96-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average200.2310.2270.229100.3420.3370.34050.4970.4820.4902.50.6630.6450.6541.250.9320.9180.9250.6251.3001.2461.2730.3121.7741.7241.74902.4342.3892.412 | |||||||
货期：3-5 working days
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