首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠硫酸乙酰肝素蛋白多糖2(HSPG2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、支气管肺泡灌洗液、尿液、组织匀浆样本中的HSPG2含量。HSPG2 即硫酸乙酰肝素蛋白聚糖 2，是细胞外基质的重要成分。它参与细胞黏附、增殖等过程。研究机制方面，其异常表达与肿瘤生长、侵袭和转移密切相关，还涉及多种疾病的病理进程，是颇具潜力的疾病诊断与治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，特别适用于体外实验模型中HSPG2表达的动态监测，例如细胞外基质相互作用研究、血管生成机制探索或组织修复模型的建立。其宽泛的样本兼容性为科研人员提供了灵活选择实验样本的条件，支持从体液到组织水平的系统性研究。为分子生物学、细胞生物学及基础医学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：RP11-132G19.2, PLC, PRCAN, SJA, SJS, SJS1, Schwartz-Jampel syndrome 1 (chondrodystrophic myotonia)|endorepellin (domain V region)|heparan sulfate proteoglycan of basement membrane|perlecan proteogly
缩写：HSPG2
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, bronchoalveolar lavage fluid, urine, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HSPG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:800 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:1600 | Average % | 100 | ||||
| Range % | 93-107 | |||||
回收率：
| The recovery of rat HSPG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-93 | ||||
| EDTA plasma (n=4) | 104 | 100-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7662.7982.7822.6805002.2772.2862.2822.1802501.5931.5621.5781.4761250.9960.9630.9800.87862.50.5330.5420.5380.43631.20.3840.3680.3760.27415.60.2710.2910.2810.17900.1010.1030.102 | ||||||
货期：3-5 working days
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