| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RNF14; ARA54; HRIHFB2038; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein |
| Entrez GeneID | 9604 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human RNF14 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于RNF14抗体的虚构参考文献示例(仅供格式参考，非真实文献)：
1. **标题**: "RNF14 regulates DNA damage response via ubiquitination of p53 in breast cancer"
**作者**: Li et al. (2022)
**摘要**: 研究利用RNF14特异性抗体揭示其在乳腺癌中通过泛素化修饰p53调控DNA损伤修复的机制，证实RNF14缺失导致基因组不稳定性增加。
2. **标题**: "Development of a novel monoclonal antibody for RNF14 and its application in prostate cancer prognosis"
**作者**: Smith et al. (2021)
**摘要**: 报道一种新型RNF14单克隆抗体的开发，并通过免疫组化证明RNF14在前列腺癌组织中的高表达与患者不良预后相关。
3. **标题**: "RNF14 interacts with AR signaling to promote androgen-dependent transcriptional activation"
**作者**: Chen & Wang (2020)
**摘要**: 通过RNF14抗体进行免疫共沉淀实验，发现RNF14与雄激素受体(AR)形成复合物，增强AR介导的转录活性，提示其在激素依赖性癌症中的潜在作用。
4. **标题**: "RNF14 antibody-based proteomics reveals its role in metabolic regulation"
**作者**: Gonzalez et al. (2023)
**摘要**: 基于RNF14抗体的蛋白质组学分析发现其参与肝脏脂代谢调控通路，敲低实验表明RNF14缺失导致脂质积累异常。
注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索。
The RNF14 (Ring Finger Protein 14) antibody is a research tool used to detect and study the RNF14 protein, an E3 ubiquitin ligase involved in ubiquitination pathways and cellular regulation. RNF14 contains a characteristic RING finger domain, which facilitates its enzymatic activity in tagging target proteins with ubiquitin for proteasomal degradation or signaling modulation. It interacts with nuclear receptors, including androgen and estrogen receptors, playing roles in transcriptional regulation, DNA repair, and hormone-responsive pathways. Dysregulation of RNF14 has been implicated in cancers (e.g., prostate, breast) and neurodegenerative disorders, making it a focus of therapeutic exploration.
RNF14 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They are validated for applications such as Western blotting, immunohistochemistry, immunoprecipitation, and immunofluorescence. Specificity is confirmed via knockdown/knockout controls, ensuring recognition of the ~40-45 kDa RNF14 protein. Researchers use these antibodies to investigate RNF14's expression patterns, post-translational modifications, and interactions with partners like HSP70 or AR/ER. Commercial antibodies often cite reactivity across human, mouse, and rat samples, though species specificity should be verified experimentally. Proper validation remains critical due to potential cross-reactivity with homologous E3 ligases like RNF4 or RNF25.
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