首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人RED蛋白(IK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IK含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，广泛应用于基础研究中的蛋白质互作分析、疾病模型构建、药物作用机制探索等方向，特别适用于癌症生物学、干细胞分化研究及分子病理学等领域的定量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：chondrosarcoma associated protein 2 ELISA Kit; CSA2 ELISA Kit; Cytokine IK ELISA Kit; IK ELISA Kit; IK cytokine down regulator of HLA II ELISA Kit; IK factor ELISA Kit; IK Gene ELISA Kit; prer protein ELISA Kit; Protein Red ELISA Kit; Protein RER ELISA Kit; RD element ELISA Kit; RED protein ELISA Kit; RED_HUMAN ELISA Kit; RER ELISA Kit
缩写：IK
Uniprot No.：Q13123
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-94 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 98-104 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 85-96 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of human IK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-94 | |||||
| EDTA plasma (n=4) | 93 | 86-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.9092.9652.9372.7638002.2152.3322.2742.1004001.4621.4871.4751.3012000.9000.9230.9120.7381000.5550.5640.5600.386500.3630.3780.3710.197250.2430.2490.2460.07200.1730.1740.174 | |||||||
货期：3-5 working days
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