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产品描述：人POTE锚蛋白域家庭成员E(POTEE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的POTEE含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础医学研究中POTEE蛋白的定量检测，可用于探索其在肿瘤微环境中的功能机制、评估不同病理状态下蛋白表达水平变化，或作为体外模型研究中细胞信号通路调控的检测工具，为肿瘤生物学、分子病理学等领域的科研工作提供标准化检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：POTEE ELISA Kit; A26C1A ELISA Kit; POTE2 ELISA Kit; POTE ankyrin domain family member E ELISA Kit; ANKRD26-like family C member 1A ELISA Kit; Prostate ELISA Kit; ovary ELISA Kit; testis-expressed protein on chromosome 2 ELISA Kit; POTE-2 ELISA Kit
缩写：POTEE
Uniprot No.：Q6S8J3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human POTEE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 93-103 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:8 | Average % | 91 | |||||
| Range % | 86-97 | ||||||
回收率：
| The recovery of human POTEE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 96-108 | |||||
| EDTA plasma (n=4) | 89 | 83-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.7862.9342.8602.7298002.2142.3412.2782.1474001.4251.4671.4461.3152000.7430.7540.7490.6181000.4560.4460.4510.320500.3270.3120.3200.189250.2160.2110.2140.08300.1320.1300.131 | |||||||
货期：3-5 working days
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