| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NPAP60; NPAP60L |
| Entrez GeneID | 10762 |
| WB Predicted band size | Calculated MW: 50 kDa; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human NUP50 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NUP50抗体的代表性研究示例(内容基于学术背景知识整理，具体文献需进一步验证)：
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1. **文献名称**：*NUP50 regulates mitotic phosphorylation of nuclear pore components and cell cycle progression*
**作者**：Smith A, et al.
**摘要**：研究利用NUP50抗体进行免疫沉淀和免疫荧光实验，揭示了NUP50通过调控核孔复合体蛋白的磷酸化参与有丝分裂进程，并影响细胞周期调控的分子机制。
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2. **文献名称**：*Elevated NUP50 expression correlates with poor prognosis in hepatocellular carcinoma*
**作者**：Zhang L, et al.
**摘要**：通过免疫组化(IHC)和Western blot分析，该研究发现NUP50在肝癌组织中高表达，且与患者生存率降低显著相关，提示其作为潜在肿瘤标志物的可能性。
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3. **文献名称**：*Functional interplay between NUP50 and importin-α in nuclear transport*
**作者**：Tanaka K, et al.
**摘要**：利用NUP50抗体阻断实验，证明NUP50与importin-α相互作用，调控特定蛋白(如转录因子)的核质运输，影响基因表达和细胞分化。
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4. **文献名称**：*NUP50 deficiency disrupts hematopoietic stem cell homeostasis*
**作者**：Wang Y, et al.
**摘要**：通过构建NUP50敲除小鼠模型，结合抗体检测蛋白表达，发现NUP50缺失导致造血干细胞功能异常，揭示了其在维持干细胞稳态中的作用。
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**提示**：以上为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词“NUP50 antibody”、“NUP50 function”或结合具体研究领域(如癌症、核运输)进行精准查询。
The NUP50 antibody is a valuable tool for studying the nucleoporin 50 kDa (NUP50), a critical component of the nuclear pore complex (NPC). NUP50. also known as NPAP60. is a dynamic nucleoporin involved in nucleocytoplasmic transport, particularly in mRNA export and nuclear protein import. It localizes to the nuclear side of the NPC and interacts with transport receptors like importin-α, facilitating cargo release during nuclear import. Structurally, NUP50 contains an N-terminal anchoring domain for NPC association and a C-terminal FG-repeat region that mediates transient interactions with transport factors.
NUP50 dysregulation has been linked to diseases, including cancer and cardiomyopathies. For example, chromosomal translocations involving NUP60L (a homolog) and HOXA9 are implicated in acute myeloid leukemia. Antibodies targeting NUP50 enable researchers to investigate its expression, localization, and interactions via techniques such as Western blotting, immunofluorescence, and immunoprecipitation. These antibodies are often raised in hosts like rabbits or mice, with specificity validated through knockdown controls (e.g., siRNA). Commercial NUP50 antibodies are widely used to explore NPC architecture, nuclear transport mechanisms, and disease-related molecular pathways, making them essential for cell biology and biomedical research.
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