首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人过氧化物酶2(PRDX2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PRDX2含量。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于基础研究中PRDX2表达水平的动态监测、疾病模型氧化损伤评估、药物干预效果分析及信号通路机制探索等方向，为细胞实验、动物模型及临床前研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PRDX2
Uniprot No.：P32119
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-108 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-102 | |||||
回收率：
| The recovery of human PRDX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.3812.4192.4002.25315001.9351.9521.9441.7977501.4271.4031.4151.2683751.0681.0591.0640.917187.50.6270.650.6390.492940.4120.4230.4180.271470.2950.280.2880.14100.1490.1450.147 | ||||||
货期：3-5 working days
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