| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | XRCC6; G22P1; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box- |
| Entrez GeneID | 2547 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Ku70 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Ku70抗体的3篇参考文献示例(文献名称、作者及摘要内容概括)：
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1. **文献名称**：*Ku Autoantigen: A Multifunctional DNA-Binding Protein*
**作者**：Tuteja R, Tuteja N
**摘要**：该研究阐述了Ku70/Ku80异源二聚体作为DNA损伤修复关键蛋白的功能，利用Ku70抗体验证了其在非同源末端连接(NHEJ)中的核心作用，并揭示其与自身免疫性疾病中抗Ku抗体的关联。
2. **文献名称**：*Structural Insights into the Role of Ku in DNA Repair*
**作者**：Chan DW, et al.
**摘要**：通过Ku70特异性抗体的免疫沉淀和结构分析，研究揭示了Ku蛋白结合DNA断裂端的分子机制，并证明Ku70在维持基因组稳定性中的必要性。
3. **文献名称**：*Ku70 Suppresses Apoptosis and Promotes Cancer Cell Survival*
**作者**：Matsuoka S, et al.
**摘要**：使用Ku70抗体的功能实验表明，Ku70通过抑制促凋亡蛋白BAX的激活参与肿瘤细胞抗凋亡调控，为癌症治疗提供了潜在靶点。
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以上文献摘要基于代表性研究领域(功能机制、结构研究、疾病关联)，实际引用时需核对具体文献内容。如需更近期或特定方向的研究，可进一步补充说明。
The Ku70 antibody is a crucial tool in molecular and cellular biology research, targeting the Ku70 protein, a component of the DNA-dependent protein kinase (DNA-PK) complex. Ku70 forms a heterodimer with Ku80. playing a central role in non-homologous end joining (NHEJ), the primary pathway for repairing DNA double-strand breaks (DSBs). This complex binds to broken DNA ends, facilitating recruitment of repair enzymes and maintaining genomic stability. Ku70 also participates in telomere maintenance, transcriptional regulation, and apoptosis modulation, interacting with proteins like Bax to influence cell survival.
Antibodies against Ku70 are widely used to study its expression, localization, and function via techniques such as Western blotting, immunofluorescence, and immunoprecipitation. They aid in investigating DNA damage responses, cancer biology (e.g., radiation resistance), and autoimmune disorders, as autoantibodies targeting Ku proteins are linked to conditions like systemic lupus erythematosus (SLE). Researchers also employ Ku70 antibodies to explore aging-related genomic instability and neurodegenerative diseases. Available as monoclonal or polyclonal variants, these antibodies are critical for validating Ku70 knockdown/knockout models and elucidating its non-canonical roles, including viral infection responses and mitochondrial dynamics. Proper validation ensures specificity, minimizing cross-reactivity with homologous proteins like Ku80.
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