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产品描述：人早期前列腺癌抗原2(EPCA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EPCA2含量。EPCA2是早期前列腺癌的潜在标志物，其研究机制涉及DNA低甲基化导致抗肿瘤免疫基因沉默，从而促进肿瘤免疫逃逸。EPCA2的表达与前列腺癌的早期发生和进展密切相关，为肿瘤的早期检测和治疗提供了新的思路。试剂盒检测范围为12.5 ng/mL-400 ng/mL，可为前列腺癌的早期分子机制研究及疗效评估提供重要依据；满足多种科研场景需求，包括体外细胞模型、动物实验或生物样本库中前列腺癌相关蛋白表达谱分析；适用于分子生物学、肿瘤学等领域的基础研究，为探索前列腺癌病理机制及潜在治疗靶点提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：EPCA2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 ng/mL-400 ng/mL
灵敏度：6.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<20% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EPCA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 82-96 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-101 | |||||
回收率：
| The recovery of human EPCA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-103 | ||||
| EDTA plasma (n=4) | 99 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average4002.2382.3452.2922001.3621.3541.3581000.6690.6510.660250.1950.2050.20012.50.0940.0970.096 | ||||||
货期：3-5 working days
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