| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | XRCC6; G22P1; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box- |
| Entrez GeneID | 2547 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Ku70 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Ku70抗体的3篇经典文献摘要示例(注：以下为模拟内容，实际文献请通过学术数据库核实)：
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1. **文献名称**：*"Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination in vivo"*
**作者**：Chen DJ, et al.
**摘要**：该研究利用Ku70基因敲除小鼠模型，证实Ku70蛋白在DNA双链断裂修复中的核心作用，但发现其缺失不影响T细胞受体基因重组过程。实验使用Ku70抗体进行免疫印迹分析，验证了蛋白表达缺失。
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2. **文献名称**：*"Autoantibodies against Ku70 in systemic lupus erythematosus: Epitope mapping and pathogenetic implications"*
**作者**：Bombardieri S, et al.
**摘要**：研究通过Ku70抗体检测系统性红斑狼疮(SLE)患者血清中的自身抗体，发现Ku70的特定抗原表位与疾病活动性相关，提示其在自身免疫反应中的潜在致病机制。
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3. **文献名称**：*"Ku70 modulates the cellular response to oxidative stress and ionizing radiation"*
**作者**：Li GC, et al.
**摘要**：该文献利用Ku70抗体进行免疫共沉淀实验，揭示Ku70通过调控氧化应激相关蛋白的稳定性，影响细胞对电离辐射的敏感性，为癌症放疗研究提供新靶点。
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如需具体文献，建议通过PubMed或Google Scholar以“Ku70 antibody”及相关关键词检索近年高被引论文。
**Background of Ku70 Antibody**
The Ku70 protein, encoded by the *XRCC6* gene, is a critical component of the DNA-dependent protein kinase (DNA-PK) complex, which plays a central role in non-homologous end joining (NHEJ), the primary pathway for repairing double-strand DNA breaks (DSBs) in mammalian cells. Ku70 forms a heterodimer with Ku80. binding to DNA ends to recruit downstream repair factors, ensuring genomic stability. Dysregulation of Ku70 is linked to genomic instability, cancer, and aging.
Ku70 antibodies are essential tools in molecular biology and cancer research, enabling the detection and quantification of Ku70 expression via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help investigate Ku70's role in DNA repair, telomere maintenance, and apoptosis. For instance, studies using Ku70 antibodies have revealed its involvement in regulating apoptotic pathways by sequestering pro-apoptotic proteins like Bax, highlighting its dual role in cell survival and death.
Additionally, Ku70 antibodies are used to explore its interactions with viral proteins and involvement in autoimmune diseases, as autoantibodies against Ku70/Ku80 are biomarkers in conditions like systemic lupus erythematosus (SLE). Research also links Ku70 deficiency to increased sensitivity to ionizing radiation and chemotherapy, underscoring its therapeutic relevance. Commercial Ku70 antibodies are typically validated for specificity across species (human, mouse, rat) and applications, ensuring reliability in diverse experimental models. Understanding Ku70's functions through antibody-based assays continues to advance insights into cancer mechanisms and DNA repair-targeted therapies.
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