首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔凝血酶原片段F1+2(F1+2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F1+2含量。兔凝血酶原片段F1+2（F1+2）是凝血酶原分子的氨基末端片段，当凝血酶被激活时释放，具有多肽特性，半衰期约90分钟。其在凝血过程中通过检测F1+2水平，可反映凝血酶的活性，是评估凝血功能的重要指标。试剂盒检测范围为0.312 pmol/mL-20 pmol/mL，常用于血栓形成机制、抗凝药物作用评估等研究；支持科研人员探究心血管疾病模型、凝血功能障碍动物实验中的F1+2动态变化；为凝血与纤溶系统调控、药物干预效果分析等基础研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：F1+2
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 pmol/mL-20 pmol/mL
灵敏度：0.078 pmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-95 | |||||
回收率：
| The recovery of rabbit F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 83-92 | ||||
| EDTA plasma (n=4) | 92 | 85-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/mlOD1OD2AverageCorrected 202.9892.7882.8892.786 102.5352.3722.4542.351 51.7811.8151.7981.695 2.51.0021.0121.0070.904 1.250.4850.5060.4960.393 0.6250.3010.3110.3060.203 0.3120.1780.1750.1770.074 00.1030.1020.103 | ||||||
货期：3-5 working days
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