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产品描述:人锌α2糖蛋白(AZGP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的AZGP1含量。AZGP1作为靶点,目前尚无足够通用信息介绍其背景。一般而言,研究可能聚焦在它在疾病发生发展中的作用机制,如在肿瘤等疾病中可能参与细胞增殖、分化等过程,后续研究或会基于此探索其作为治疗靶点的潜力和相关药物研发。试剂盒检测范围为7.8 ng/mL-500 ng/mL,适用于基础科研中AZGP1蛋白表达动力学研究、疾病模型样本的分子标志物检测,以及代谢通路调控机制探索等场景,为癌症生物学、免疫代谢研究及药物开发提供可靠的数据支持本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Alpha 2 glycoprotein 1 zinc ELISA Kit; Alpha 2 glycoprotein 1 zinc binding ELISA Kit; Alpha 2 glycoprotein zinc ELISA Kit; Azgp1 ELISA Kit; ZA2G ELISA Kit; ZA2G_HUMAN ELISA Kit; ZAG ELISA Kit; Zinc alpha 2 glycoprotein precursor ELISA Kit; Zinc-alpha-2-glycoprotein ELISA Kit; Zn alpha 2 glycoprotein ELISA Kit; Zn alpha 2 GP ELISA Kit; Zn alpha2 glycoprotein ELISA Kit; Zn-alpha-2-glycoprotein ELISA Kit; Zn-alpha-2-GP ELISA Kit; ZNGP1 ELISA Kit
缩写:AZGP1
Uniprot No.:P25311
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:7.8 ng/mL-500 ng/mL
灵敏度:1.95 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human AZGP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 97 | ||||
Range % | 92-105 | |||||
1:200 | Average % | 97 | ||||
Range % | 92-103 | |||||
1:400 | Average % | 92 | ||||
Range % | 83-98 | |||||
1:800 | Average % | 90 | ||||
Range % | 84-99 |
回收率:
The recovery of human AZGP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 98 | 92-103 | ||||
EDTA plasma (n=4) | 93 | 85-98 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected5002.5242.3252.4252.2392502.0231.9511.9871.8011251.5751.5311.5531.36762.51.1341.0861.1100.92431.20.7160.6590.6880.50215.60.4080.4040.4060.2207.80.2590.2410.2500.06400.1920.1790.186 |
货期:3-5 working days
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