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产品描述：猪肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CK-MB含量。CK - MB即肌酸激酶同工酶，主要存在于心肌中。其水平变化可反映心肌损伤情况，是诊断心肌梗死等疾病的重要指标。研究机制上，可通过检测血液中其含量变化，辅助判断病情严重程度、评估治疗效果等。试剂盒检测范围为2.35 mU/mL-150 mU/mL，适用于猪心血管疾病基础研究、心肌损伤模型验证、药物心脏毒性评估及疾病机制探索等科研领域。研究人员可通过高效分析不同处理条件下心肌损伤的动态变化，为动物模型研究及生物医学实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CK-MB
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：2.35 mU/mL-150 mU/mL
灵敏度：0.58 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-104 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 87-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-100 | |||||
回收率：
| The recovery of pig CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-105 | ||||
| EDTA plasma (n=4) | 92 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected1502.8022.7912.7972.607752.3602.3142.3372.14737.51.7051.7161.7111.52118.751.1271.1131.1200.9309.370.6150.6020.6090.4194.680.4240.4380.4310.2412.350.3250.3190.3220.13200.1910.1890.190 | ||||||
货期：3-5 working days
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