首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：大肠杆菌宿主残留蛋白(E.coli P)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的E.coli P含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，可有效监测生物制品生产过程中的宿主蛋白残留；满足生物制药工艺开发、重组蛋白纯化效果验证、转基因生物样本分析等科研场景；适用于实验室规模的生产监控、生物工程研究中的质量评估以及基础科研中的宿主蛋白残留分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：E.coli P
种属：Escherichia Coli
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Microbiology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of escherichia coli protein in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of escherichia coli protein spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 96 | 89-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.0482.1112.0801.929 51.7691.8101.7901.639 2.51.2361.3141.2751.124 1.250.7520.7630.7580.607 0.6250.4190.4240.4220.271 0.3120.3060.3150.3110.160 0.1560.2370.2410.2390.088 00.1500.1520.151 | ||||||
货期：3-5 working days
×
