首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人连蛋白(zonulin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的zonulin含量。Zonulin是一种调节肠道紧密连接的蛋白质，其水平升高与肠道通透性增加和自身免疫性疾病风险相关。研究显示，Zonulin通过调节肠道屏障功能，影响肠道微生物群和免疫系统，从而在多种疾病的发生发展中扮演重要角色。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该蛋白作为调节肠道屏障通透性的关键分子，广泛参与肠道炎症、自身免疫性疾病及代谢综合征等病理过程的研究。试剂盒支持血清、血浆及组织匀浆样本分析，通过高灵敏度检测帮助科研人员深入探究肠道屏障功能失调机制、疾病模型构建或药物干预效果评估。适用于基础医学研究、生物标志物筛选及分子病理学等科研领域，为探索zonulin在肠道健康与疾病中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：zonulin
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human zonulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 93-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human zonulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-101 | ||||
| EDTA plasma (n=4) | 97 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.1482.0662.1072.008201.4551.3531.4041.305100.8670.8950.8810.78250.5570.5710.5640.4652.50.3930.3750.3840.2851.250.2640.2760.2700.1710.6250.1950.1990.1970.09800.0980.0990.099 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human zonulin antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-labeled zonulin antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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