首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肺表面活性蛋白D(SP-D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SP-D含量。小鼠肺表面活性蛋白D（SP-D）是一种在肺泡上皮细胞中表达的蛋白质，具有调节肺泡表面张力和参与宿主防御功能。研究显示，SP-D在急性肺损伤和肺气肿等疾病中发挥重要作用，其缺失可能导致肺功能异常和疾病敏感性增加。通过基因编辑技术构建SP-D敲除小鼠模型，有助于深入理解SP-D在肺生理和疾病发生发展中的作用机制。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品适用于基础科研中探索肺部疾病模型（如急性肺损伤、肺炎或纤维化）的分子机制，评估体外细胞模型中SP-D的分泌动态，或通过组织样本分析病理状态下SP-D的表达变化，为呼吸道免疫调控研究提供高灵敏度的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sftpd ELISA Kit; Sftp4 ELISA Kit; Pulmonary surfactant-associated protein D ELISA Kit; PSP-D ELISA Kit; SP-D ELISA Kit; Lung surfactant protein D ELISA Kit
缩写：SP-D
Uniprot No.：P50404
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 91-101 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of mouse SP-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 98 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.1352.0522.0941.923501.7621.6761.7191.548251.3171.2341.2761.10512.50.8440.8390.8420.6716.250.5690.5270.5480.3773.120.3360.3190.3280.1571.560.2110.2070.2090.03800.1730.1690.171 | ||||||
货期：3-5 working days
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