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产品描述：犬胰岛素样生长因子1(IGF1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF1含量。IGF1即胰岛素样生长因子1，在细胞生长、增殖和分化等生理过程中起关键作用。其背景在于与生长发育密切相关。研究机制方面，主要通过与受体结合激活下游信号通路，影响细胞生命活动，在肿瘤、代谢疾病等研究中备受关注。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，适用于犬类代谢疾病机制研究、生长发育评估、营养干预实验以及内分泌相关动物模型的数据采集，为探索IGF1在犬类生理病理过程中的调控网络提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGF1 ELISA Kit; IGFIAInsulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写：IGF1
Uniprot No.：P33712
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.8 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IGF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 100-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-99 | |||||
回收率：
| The recovery of dog IGF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-100 | ||||
| EDTA plasma (n=4) | 87 | 80-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 20002.5442.4872.5162.351 10001.9642.0061.9851.820 5001.3051.3941.3501.185 2500.6900.7380.7140.549 1250.4480.4820.4650.300 62.50.3610.3390.3500.185 31.250.2570.2450.2510.086 00.1620.1680.165 | ||||||
货期：3-5 working days
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