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产品描述：牛补体片断5a(C5a)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C5a含量。C5a是补体系统激活后产生的重要炎性介质。其背景在于它参与多种生理与病理过程。研究机制上，C5a能与受体结合，介导炎症细胞趋化、活化，释放炎症因子，在感染、自身免疫病、肿瘤等疾病中发挥作用，是潜在治疗靶点。试剂盒检测范围为4.69 ng/mL-300 ng/mL，该产品适用于动物免疫机制研究、炎症性疾病模型构建、疫苗效力评估等科研领域，尤其为反刍动物感染性疾病的免疫病理分析提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C5a
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：4.69 ng/mL-300 ng/mL
灵敏度：1.17 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 88-93 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of bovine C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-103 | ||||
| EDTA plasma (n=4) | 90 | 86-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 3002.6982.4892.5942.487 1502.1322.2452.1892.082 751.5761.7061.6411.534 37.51.1331.0501.0920.985 18.750.7140.6770.6960.589 9.380.4400.4660.4530.346 4.690.2780.2560.2670.160 00.1060.1070.107 | ||||||
货期：3-5 working days
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