首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠氧化高密度脂蛋白(Ox-HDL)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Ox-HDL含量。Ox - HDL是氧化型高密度脂蛋白。在生理背景下，HDL氧化后功能改变。研究机制方面，其可影响胆固醇逆向转运等过程，还能与细胞表面受体相互作用，引发一系列细胞内信号转导变化，与动脉粥样硬化等疾病的发生发展密切相关。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于心血管疾病动物模型研究、抗氧化药物疗效评估、氧化应激相关机制探索等科研领域。通过检测不同样本类型中的 Ox-HDL 含量，可为脂蛋白氧化损伤机制研究及潜在干预策略开发提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Ox-HDL
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ox-HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 94-104 | |||||
| 1:800 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of mouse Ox-HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-97 | ||||
| EDTA plasma (n=4) | 99 | 95-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average10000.0970.0960.0975000.2670.2630.2652500.4660.4540.4601250.6910.6870.68962.51.0801.0911.08631.251.3911.4121.40215.61.5041.6061.55501.8131.7111.762 | ||||||
货期：3-5 working days
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