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产品描述：人肾上腺髓质素前体(Pro-ADM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、组织匀浆样本中的Pro-ADM含量。人肾上腺髓质素前体（Pro-ADM）是一种由多种组织产生的肽，具有稳定微循环和防止内皮通透性的作用。其在脓毒症、下呼吸道感染等疾病中显示出作为治疗监测和风险评估的潜力，其水平与疾病严重程度和后续不良事件的可能性相关。试剂盒检测范围为0.312 pmol/L-20 pmol/L，该产品支持多种生物样本类型，能够满足基础医学研究中疾病机制探索、药物作用评估或生物标志物筛选等需求，例如通过检测循环系统中Pro - ADM的动态变化，为心血管疾病或全身性炎症相关研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Pro-ADM
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine, tissue homogenates
检测范围：0.312 pmol/L-20 pmol/L
灵敏度：0.078 pmol/L
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:800 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:1600 | Average % | 92 | ||||
| Range % | 82-105 | |||||
回收率：
| The recovery of human Pro-ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-100 | ||||
| EDTA plasma (n=4) | 101 | 90-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/LOD1OD2AverageCorrected203.0522.9523.0022.860102.5872.4762.5322.39051.8191.7041.7621.6202.51.1251.1091.1170.9751.250.7020.6840.6930.5510.6250.4060.3890.3980.2560.3120.2730.2580.2660.12400.1470.1370.142 | ||||||
货期：3-5 working days
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