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Rabbit Monoclonal hHR23A Antibody

  • 中文名： hHR23A抗体
  • 别    名： RAD23A; UV excision repair protein RAD23 homolog A; HR23A; hHR23A
货号: IPDX22390
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRAD23A; UV excision repair protein RAD23 homolog A; HR23A; hHR23A
Entrez GeneID5886
WB Predicted band sizeCalculated MW: 40 kDa; Observed MW: 52 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenA synthetic peptide of human hHR23A
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于hHR23A抗体的3篇参考文献及其摘要信息：

1. **"hHR23A facilitates the repair of UV-induced DNA damage through interaction with XPC"**

- **作者**: Sugasawa, K., Okamoto, T., Shimizu, Y., et al.

- **摘要**: 研究揭示了hHR23A通过与核苷酸切除修复(NER)关键因子XPC结合，参与紫外线诱导的DNA损伤修复过程，并利用特异性抗体验证了两者的相互作用及定位。

2. **"Rad23 and the DNA damage response: Coordination between proteasomal degradation and DNA repair"**

- **作者**: Dantuma, N.P., Acs, K., Luijsterburg, M.S.

- **摘要**: 探讨hHR23A在DNA损伤应答中的双重功能，既通过泛素-蛋白酶体系统调控错误折叠蛋白降解，又通过稳定XPC复合体促进DNA修复，研究使用抗体进行蛋白质互作分析。

3. **"Ubiquitin recognition by hHR23A: Structural insights into proteasomal substrate delivery"**

- **作者**: Walters, K.J., Kleijnen, M.F., Goh, A.M., et al.

- **摘要**: 通过结构生物学和抗体免疫沉淀实验，阐明hHR23A通过泛素相关结构域(UBA)识别多聚泛素链，并介导底物向蛋白酶体的定向运输。

4. **"Regulation of USP14 by hHR23A: Implications for protein quality control"**

- **作者**: Kim, H.T., Goldberg, A.L., et al.

- **摘要**: 发现hHR23A与去泛素化酶USP14相互作用，调控蛋白酶体活性及错误折叠蛋白的清除，研究通过抗体阻断实验验证了hHR23A在泛素依赖性降解中的作用。

以上文献均聚焦于hHR23A在DNA修复、泛素-蛋白酶体途径中的功能，并涉及抗体的实验验证。

背景信息

The hHR23A antibody is a tool used to detect and study the human homolog of Rad23A (hHR23A), a protein critically involved in nucleotide excision repair (NER) and ubiquitin-mediated proteolysis. hHR23A, encoded by the RAD23A gene, functions as a shuttle factor in the ubiquitin-proteasome system (UPS), facilitating the degradation of polyubiquitinated proteins. It contains an N-terminal ubiquitin-like (UBL) domain and two ubiquitin-associated (UBA) domains, enabling interactions with the 26S proteasome and ubiquitinated substrates. hHR23A also partners with XPC (xeroderma pigmentosum complementation group C) to form the XPC-hHR23B complex, which plays a pivotal role in recognizing DNA lesions during global genome NER. While hHR23B is more studied in DNA repair, hHR23A shares overlapping functions but may have distinct roles in protein quality control and stress responses. Antibodies against hHR23A are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, localization, and interactions in cellular contexts. Researchers employ these antibodies to explore hHR23A's involvement in cancer (e.g., dysregulation in proteostasis), neurodegenerative diseases (e.g., aggregation-prone protein clearance), and UV-induced DNA damage repair mechanisms. The antibody's specificity and reliability make it essential for dissecting molecular pathways linked to genomic stability and protein homeostasis.

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