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产品描述：大鼠5` -核苷酸酶，胞膜(NT5E/CD73)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的NT5E/CD73含量。NT5E/CD73是一个重要靶点，它在腺苷代谢中起关键作用。其高表达可促进肿瘤微环境免疫抑制。研究显示，通过抑制CD73活性阻断腺苷生成，或能逆转免疫抑制，增强机体抗肿瘤免疫反应，当前围绕它的靶向治疗研究是热门方向。试剂盒检测范围为0.625 mIU/mL-40 mIU/mL，适用于评估CD73在肿瘤微环境、神经退行性疾病模型或代谢性疾病研究中的表达特征，也可用于药物干预实验中的生物标志物检测。为研究腺苷信号通路及其相关疾病机制提供可靠的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
缩写：NT5E/CD73
Uniprot No.：P21588
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：0.625 mIU/mL-40 mIU/mL
灵敏度：0.156 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 82-95 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-101 | |||||
回收率：
| The recovery of rat CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-101 | ||||
| EDTA plasma (n=4) | 91 | 86-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected402.5042.4162.4602.358201.9331.8241.8791.777101.3861.2831.3351.23350.8140.7490.7820.6802.50.4240.3970.4110.3091.250.2280.2130.2210.1190.6250.1540.1450.1500.04800.1030.1010.102 | ||||||
货期：3-5 working days
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