| WB | 1/500-1/1000 | Human,Mouse,Rat,Hamster |
| IF | 1/20 | Human,Mouse,Rat,Hamster |
| IHC | 咨询技术 | Human,Mouse,Rat,Hamster |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Hamster |
| FCM | 咨询技术 | Human,Mouse,Rat,Hamster |
| Elisa | 咨询技术 | Human,Mouse,Rat,Hamster |
| Aliases | TUBG1; TUBG; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1; GCP-1 |
| Entrez GeneID | 7283 |
| WB Predicted band size | Calculated MW: 51 kDa; Observed MW: 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Hamster |
| Immunogen | Recombinant protein of human gamma Tubulin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于gamma Tubulin抗体的3篇参考文献及其摘要概述：
1. **"Gamma-tubulin is a component of the centrosome and a conserved component of microtubule organizing centers"**
*作者：Joshi H.C., Palacios M.J., McNamara L., Cleveland D.W.*
**摘要**：该研究利用gamma Tubulin抗体在哺乳动物细胞中定位，证实gamma Tubulin是中心体的核心成分，并揭示其通过调控微管成核参与细胞分裂和胞器排布。
2. **"Gamma-tubulin in mammalian cells: Localization and functional analysis using specific antibodies"**
*作者：Oakley B.R., Oakley C.E., Yoon Y.S., Jung M.K.*
**摘要**：通过开发特异性抗体，作者发现gamma Tubulin广泛分布于中心体和纺锤体，并通过抗体干扰实验证明其缺失会导致微管组织紊乱，影响有丝分裂进程。
3. **"Purification and characterization of gamma-tubulin-containing complexes from Drosophila embryos"**
*作者：Moritz M., Braunfeld M.B., Sedat J.W., Alberts B., Agard D.A.*
**摘要**：研究利用gamma Tubulin抗体从果蝇胚胎中分离出gamma-TuRC复合物，解析其三维结构，并证明该复合物通过招募α/β微管蛋白二聚体启动微管组装。
4. **"The structure and function of gamma-tubulin complexes"**
*作者：Kollman J.M., Polka J.K., Zelter A., Davis T.N., Agard D.A.*
**摘要**：这篇综述整合多篇抗体研究数据，系统总结gamma-TuRC复合物的结构、动态调控机制及其在疾病(如癌症)中的潜在作用靶点。
以上文献均聚焦于gamma Tubulin抗体的应用，涵盖定位、功能验证及复合物解析，为相关研究提供关键实验依据。
Gamma tubulin is a conserved member of the tubulin protein family, primarily recognized for its critical role in microtubule nucleation and organization. Unlike α- and β-tubulins that form microtubule polymers, gamma tubulin functions as a core component of the γ-tubulin ring complex (γ-TuRC), which acts as a template for microtubule assembly. It localizes to centrosomes, spindle pole bodies, and other microtubule-organizing centers (MTOCs), where it initiates the polymerization of α/β-tubulin heterodimers. This makes gamma tubulin indispensable for mitotic spindle formation, cell division, and maintaining cytoskeletal architecture.
Antibodies targeting gamma tubulin are widely used as essential tools in cell biology research. They enable the visualization and quantification of gamma tubulin in techniques such as immunofluorescence, Western blotting, and immunoprecipitation. These antibodies help identify MTOCs, assess centrosome integrity, and study defects in microtubule dynamics linked to mitotic errors, developmental disorders, or cancer. Commonly generated in hosts like rabbits or mice, gamma tubulin antibodies are often validated using knockout controls or siRNA knockdown to ensure specificity. In experimental settings, they also serve as loading controls in protein analysis due to their consistent expression levels across cell cycle phases. Researchers rely on these antibodies to explore mechanisms underlying cell division, cilia formation, and diseases associated with centrosome amplification or microtubule dysregulation.
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