首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠溶菌酶(LZM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LZM含量。LZM（溶菌酶）它是一种天然抗菌物质，广泛存在于多种生物体内。研究机制上，其能特异性地作用于细菌细胞壁肽聚糖，破坏细胞壁结构，导致细菌溶解死亡，在医药、食品等领域有潜在应用价值。试剂盒检测范围为0.78 ng/mL-50 ng/mL，该产品可广泛应用于小鼠感染性疾病模型、免疫系统功能评估、炎症机制研究以及药物干预实验中溶菌酶动态变化的检测，为科研人员探究先天免疫应答机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LZM
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 94-101 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 87-100 | |||||
回收率：
| The recovery of mouse LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-100 | ||||
| EDTA plasma (n=4) | 91 | 85-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.5232.4562.4902.300251.8451.8691.8571.66712.51.2671.3651.3161.1266.250.8020.8290.8160.6263.120.4830.4860.4850.2951.560.3450.3620.3540.1640.780.2660.2690.2680.07800.1920.1880.190 | ||||||
货期：3-5 working days
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