首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠凋亡相关因子(FAS/CD95)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FAS含量。FAS是重要的细胞表面受体。其背景上，在细胞凋亡调控等生理过程中意义重大。研究机制方面，FAS与配体结合后，会激活级联反应，诱导细胞凋亡，在肿瘤、自身免疫病等疾病研究里，是潜在治疗靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于体外细胞凋亡机制研究、药物干预效果评估或动物模型中FAS信号通路的动态监测，为探索细胞程序性死亡调控机制、免疫微环境分析及靶向治疗开发提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fas; Apt1; Tnfrsf6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD antigen CD95
缩写：FAS
Uniprot No.：P25446
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 97-108 | ||||||
| 1:2 | Average % | 106 | |||||
| Range % | 97-110 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 92-104 | ||||||
回收率：
| The recovery of mouse FAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 95-107 | |||||
| EDTA plasma (n=4) | 100 | 89-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected402.9652.8482.9072.737202.6582.5922.6252.455102.1662.0792.1231.95351.5021.4781.4901.3202.50.8320.8440.8380.6681.250.5510.5430.5470.3770.6250.3290.3190.3240.15400.1720.1680.170 | |||||||
货期：3-5 working days
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