| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DOC2; DOC-2 |
| Entrez GeneID | 1601 |
| WB Predicted band size | Calculated MW: 82 kDa; Observed MW: 96 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human DAB2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于DAB2抗体的研究文献示例(信息基于公开研究主题概括，具体内容建议通过学术数据库核实)：
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1. **文献名称**: *"Disabled-2 modulates TGF-β signaling in ovarian cancer cells"*
**作者**: Hocevar BA 等
**摘要**: 研究利用DAB2抗体进行Western blot和免疫荧光分析，发现DAB2通过调控TGF-β/Smad通路抑制卵巢癌细胞迁移，提示其作为肿瘤抑制因子的作用机制。
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2. **文献名称**: *"DAB2 suppresses prostate tumorigenesis through antagonizing Wnt/β-catenin signaling"*
**作者**: Zhou J 等
**摘要**: 通过免疫组化及DAB2抗体检测，证明DAB2在前列腺癌中低表达，并揭示其通过抑制Wnt通路活性阻碍肿瘤进展，为靶向治疗提供依据。
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3. **文献名称**: *"DAB2 regulates epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma"*
**作者**: Xu Y 等
**摘要**: 研究采用DAB2抗体进行蛋白定位分析，发现DAB2缺失促进肝癌细胞EMT过程，揭示其通过E-cadherin调控抑制转移的分子机制。
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**提示**：如需具体文献，建议在PubMed或Google Scholar检索关键词 **"DAB2 antibody"** 或 **"Disabled-2 immunohistochemistry"**，并筛选近五年高被引论文以获取最新实验方法与应用案例。
DAB2 (Disabled-2) is a cytosolic adaptor protein encoded by the *DAB2* gene, initially identified for its role in receptor-mediated endocytosis and cell signaling regulation. It interacts with components of clathrin-coated pits, including AP-2 and myosin VI, facilitating cargo internalization. Beyond endocytosis, DAB2 acts as a tumor suppressor by modulating key signaling pathways such as TGF-β, Wnt, and MAPK. It inhibits cell proliferation by sequestering signaling molecules (e.g., GRB2) and promoting SMAD activation in TGF-β signaling. DAB2 expression is frequently downregulated in cancers (e.g., ovarian, breast, prostate) due to epigenetic silencing or loss of heterozygosity, correlating with tumor progression and metastasis.
DAB2 antibodies are essential tools for studying its expression, localization, and function. They enable detection of two major isoforms (p96 and p67) via techniques like Western blot, immunohistochemistry, and immunofluorescence. Commonly used clones (e.g., H-56. C-18) target specific epitopes, aiding research on DAB2's role in epithelial-mesenchymal transition (EMT), cell adhesion, and therapeutic responses. These antibodies are pivotal in exploring DAB2's dual roles in cellular homeostasis and disease pathogenesis, particularly in cancer and developmental contexts.
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