首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子蛋白聚糖(ACAN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ACAN含量。ACAN即聚集蛋白聚糖，是细胞外基质的重要组成部分。它参与维持组织弹性和抗压性等。研究机制方面，其异常表达与骨关节炎、肿瘤等疾病发生发展相关，如调控细胞增殖、迁移，影响微环境，目前围绕它的干预手段是研究热点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，本试剂盒适用于软骨代谢机制、关节疾病模型等基础研究领域。该产品适用于体外评估软骨细胞分泌功能、病理模型中的基质代谢动态监测，或生物材料对软骨再生的影响等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACAN ELISA Kit; AGC1 ELISA Kit; Aggrecan core protein ELISA Kit; Cartilage-specific proteoglycan core protein ELISA Kit; CSPCP ELISA Kit; Fragment ELISA Kit
缩写：ACAN
Uniprot No.：Q28670
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ACAN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 90-103 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 82-94 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 82-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of rabbit ACAN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-94 | ||||
| EDTA plasma (n=4) | 103 | 93-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.0202.1212.0711.944201.9021.8461.8741.747101.5701.5241.5471.42051.2381.2001.2191.0922.50.9550.9340.9450.8181.250.5320.5450.5390.4120.6250.3150.3090.3120.18500.1280.1260.127 | ||||||
货期：3-5 working days
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