首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔补体蛋白3(C3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的C3含量。C3作为靶点在生物医学领域受关注。它是补体系统关键成分。研究表明，C3参与免疫反应与炎症进程，异常激活影响多种疾病发展。针对其的研究机制聚焦在阻断异常激活途径，期望开发出能治疗相关疾病的疗法。试剂盒检测范围为27.3 ng/mL-7000 ng/mL，适用于兔类动物模型中的免疫调控研究，如感染性疾病机制探索、自身免疫病理分析或炎症相关药物开发中的生物标志物检测，为科研人员提供标准化的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
缩写：C3
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：27.3 ng/mL-7000 ng/mL
灵敏度：27.3 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 97 | ||||
| Range % | 91-110 | |||||
| 1:2000 | Average % | 89 | ||||
| Range % | 82-99 | |||||
| 1:4000 | Average % | 95 | ||||
| Range % | 85-99 | |||||
| 1:8000 | Average % | 91 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of rabbit C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 82-97 | ||||
| EDTA plasma (n=4) | 97 | 87-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average70000.1960.1890.19317500.3780.3720.375437.50.8120.8050.809109.41.4921.5011.49727.32.0122.0032.00802.9132.8972.905 | ||||||
货期：3-5 working days
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