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产品描述：山羊黄体生成素(LH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LH含量。LH即促黄体生成素，是腺垂体分泌的促性腺激素。其在调节性腺功能方面起关键作用，可刺激卵巢排卵和黄体形成，促进雄激素合成及精子生成。目前对其分泌调控、与相关疾病关系等机制是研究热点，以助解决生殖等方面问题。试剂盒检测范围为0.3 mLU/mL-60 mLU/mL，适用于山羊繁殖生理机制研究、卵巢功能评估、生殖内分泌调控实验以及繁殖障碍模型的建立等科研场景，例如通过动态监测发情周期中LH浓度波动，探究排卵诱导机制或评估激素干预效果本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LH
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.3 mLU/mL-60 mLU/mL
灵敏度：0.15 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 90-104 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-103 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 88-93 | |||||
回收率：
| The recovery of goat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-101 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2Average 600.1490.1440.147 150.2890.2980.294 4.80.5200.5270.524 1.20.8610.8650.863 0.31.4111.4161.414 02.2132.2122.213 | ||||||
货期：3-5 working days
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