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产品描述：山羊白介素12(IL-12/P40)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IL12B含量。IL12B是重要的靶点。IL - 12B编码p40亚基，与p35共同组成IL - 12，还能和p19组成IL - 23。其研究机制多围绕IL - 12和IL - 23介导的免疫反应，在自身免疫病、肿瘤等疾病治疗研究中受关注，通过调节免疫来探索潜在疗法。试剂盒检测范围为9.38 pg/mL-600 pg/mL，适用于科研领域探究宿主抗感染免疫机制、疫苗效力评估或炎症性疾病模型中细胞因子网络的动态变化，例如可用于研究山羊布鲁氏菌病、口蹄疫等传染性疾病过程中的免疫激活状态，或在体外实验中评估免疫刺激剂对IL - 12/P40分泌水平的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL12B ELISA Kit; Interleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
缩写：IL12B
Uniprot No.：P68221
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：9.37 pg/mL-600 pg/mL
灵敏度：2.34 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-102 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 82-97 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-105 | |||||
回收率：
| The recovery of goat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-90 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 6001.9741.9421.9581.857 3001.3311.3621.3471.246 1500.9120.9240.9180.817 750.5870.6120.6000.499 37.50.4010.3880.3950.294 18.750.2950.3090.3020.201 9.370.2140.2250.2200.119 00.1020.1000.101 | ||||||
货期：3-5 working days
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