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产品描述：人中性粒细胞防御素1-3(HNP1-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HNP1-3含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于探索感染性疾病机制、免疫调控研究、炎症相关疾病模型建立等科研场景，例如脓毒症、类风湿性关节炎或肿瘤微环境中的免疫应答研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：HNP1-3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HNP1-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 85-106 | |||||
回收率：
| The recovery of human HNP1-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 95 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.8002.7882.7942.692501.7651.7431.7541.652251.2151.2031.2091.10712.50.6980.6780.6880.5866.250.3620.3550.3590.2573.120.2620.2460.2540.1521.560.1690.1560.1630.06100.1040.1000.102 | ||||||
货期：3-5 working days
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