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产品描述：人脂氧素A4(LXA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、组织匀浆、细胞裂解物样本中的LXA4含量。LXA4（脂氧素A4）是一种内源性抗炎和免疫调节介质，由花生四烯酸通过脂氧合酶作用合成。它通过抑制炎症细胞、调节促炎和抗炎因子平衡，限制炎症损伤，在多种生理和病理过程中发挥重要作用。试剂盒检测范围为0.625 pg/mL-40 pg/mL，该产品可用于体外研究炎症相关信号通路、评估细胞模型或动物模型中LXA4的动态变化，或探索其在组织微环境调控中的作用，为炎症性疾病机制研究提供工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LXA4
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates
检测范围：0.625 pg/mL-40 pg/mL
灵敏度：0.156 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LXA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of human LXA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 87-99 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected402.4332.3742.4042.265201.9471.8791.9131.774101.2561.3071.2821.14350.7870.8110.7990.6602.50.5460.5220.5340.3951.250.3920.4010.3970.2580.6250.2460.2550.2510.11200.1350.1420.139 | ||||||
货期：3-5 working days
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