| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RSN; CLIP; CYLN1; CLIP170; CLIP-170 |
| Entrez GeneID | 6249 |
| WB Predicted band size | Calculated MW: 162 kDa; Observed MW: 170 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human CLIP170 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于CLIP170抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *CLIP-170 links endocytic vesicles to microtubules*
**作者**: Pierre, P. et al. (1992)
**摘要**: 该研究首次描述CLIP170作为微管结合蛋白，利用特异性抗体证实其在胞内运输中的作用，发现CLIP170通过连接内吞囊泡与微管网络，调控早期内体运输过程。
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2. **文献名称**: *Dynamic behavior of CLIP-170 on microtubules in living cells*
**作者**: Lansbergen, G. et al. (2003)
**摘要**: 通过抗CLIP170抗体的免疫荧光成像，作者发现CLIP170通过“捕获-释放”机制动态结合微管正端，并揭示其在微管动态组装和细胞极性建立中的关键作用。
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3. **文献名称**: *The microtubule plus-end-tracking protein CLIP-170 interacts with the Arp1 subunit of the dynactin complex*
**作者**: Akhmanova, A. et al. (2001)
**摘要**: 该研究使用CLIP170抗体进行免疫共沉淀实验，证明CLIP170与动力蛋白复合物(dynactin)的Arp1亚基相互作用，阐明其在连接微管与膜泡运输中的分子机制。
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4. **文献名称**: *Reconstitution of a microtubule plus-end tracking system in vitro*
**作者**: Bieling, P. et al. (2007)
**摘要**: 利用抗CLIP170抗体验证其在体外重组系统中的定位，发现CLIP170与EB1协同促进微管正端追踪复合物形成，为研究微管动态调控提供了实验模型。
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以上文献均涉及CLIP170抗体的应用，涵盖功能验证、动态行为及分子互作机制等领域。
The CLIP170 antibody is a crucial tool in studying the role of Cytoplasmic Linker Protein 170 (CLIP170), a key regulator of microtubule dynamics in eukaryotic cells. CLIP170. a member of the CLIP family (CLIP170. CLIP115), is a ~170 kDa protein characterized by N-terminal CAP-Gly domains that bind microtubules and C-terminal coiled-coil regions mediating homodimerization. It functions as a microtubule plus-end tracking protein (+TIP), facilitating microtubule interactions with cellular structures like the Golgi apparatus or chromosomes during mitosis. CLIP170 also participates in cargo transport, cell polarization, and mitotic spindle assembly by linking microtubules to dynein/dynactin complexes.
Antibodies targeting CLIP170 enable researchers to investigate its localization, expression, and interactions in processes such as cell division, intracellular trafficking, and cytoskeletal reorganization. They are widely used in techniques like immunofluorescence (to visualize +TIP comet structures), Western blotting, and immunoprecipitation. Dysregulation of CLIP170 has been implicated in cancers (e.g., metastasis) and neurodegenerative disorders, making these antibodies valuable in disease mechanism studies. Commercial CLIP170 antibodies are often raised against specific epitopes, such as the N-terminal region, and validated for cross-reactivity in human, mouse, or rat models. Their application has significantly advanced understanding of microtubule-mediated cellular functions and pathology.
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