| WB | 1/500-1/1000 | Human,Mouse,Rat,Hamster |
| IF | 咨询技术 | Human,Mouse,Rat,Hamster |
| IHC | 咨询技术 | Human,Mouse,Rat,Hamster |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Hamster |
| FCM | 咨询技术 | Human,Mouse,Rat,Hamster |
| Elisa | 咨询技术 | Human,Mouse,Rat,Hamster |
| Aliases | ATP-dependent Clp protease ATP-binding subunit clpX-like; mitochondrial; CLPX |
| Entrez GeneID | 10845 |
| WB Predicted band size | Calculated MW: 69 kDa; Observed MW: 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Hamster |
| Immunogen | A synthetic peptide of human CLPX |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及CLPX抗体的代表性文献摘要：
1. **文献名称**: "Role of the mitochondrial protease CLPX in neuronal homeostasis"
**作者**: Smith J, et al.
**摘要**: 研究通过Western blot和免疫荧光技术，使用特异性CLPX抗体验证了该蛋白酶在小鼠神经元线粒体中的定位，发现CLPX缺失导致错误折叠蛋白累积，提示其在神经退行性疾病中的潜在作用。
2. **文献名称**: "Development of monoclonal antibodies against human CLPX for cancer biomarker studies"
**作者**: Chen L, et al.
**摘要**: 报道了一种新型CLPX单克隆抗体的制备，通过ELISA和免疫组化验证其高特异性，并发现CLPX在结肠癌组织中的表达显著高于正常组织，提示其作为肿瘤标志物的可能性。
3. **文献名称**: "CLPX regulates mitochondrial metabolism through ISC assembly"
**作者**: Wang Y, et al.
**摘要**: 利用CLPX抗体进行免疫共沉淀实验，揭示了CLPX通过调控铁硫簇(ISC)组装影响线粒体能量代谢的分子机制，为代谢综合征研究提供了新视角。
注：以上为模拟文献，实际研究中建议通过PubMed或Web of Science以"CLPX antibody"为关键词检索真实文献。典型相关研究多集中于CLPX在蛋白质质量控制、线粒体疾病及癌症领域的机制探索。
The CLPX antibody is a research tool designed to detect and study the mitochondrial caseinolytic protease X (CLPX) protein, a key component of the ATP-dependent Clp protease complex. CLPX, a member of the AAA+ ATPase family, functions as a regulatory subunit that works in tandem with the proteolytic subunit CLPP. This complex plays a critical role in mitochondrial protein quality control by selectively degrading misfolded or damaged proteins, thereby maintaining proteostasis. CLPX utilizes ATP hydrolysis to unfold substrate proteins, enabling their degradation by CLPP.
Research on CLPX has gained attention due to its involvement in mitochondrial metabolism, stress responses, and diseases such as neurodegenerative disorders, cancer, and Perrault syndrome—a rare genetic condition linked to CLPX mutations. The CLPX antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate protein expression, localization, and interactions. Its development has facilitated insights into CLPX's molecular mechanisms, including its role in heme biosynthesis, iron-sulfur cluster assembly, and mitochondrial dynamics. Studies using CLPX antibodies have also explored its potential as a therapeutic target, particularly in cancers with dysregulated mitochondrial proteostasis. Validation of these antibodies typically includes knockout controls to ensure specificity, given the structural similarities among AAA+ ATPases.
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