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产品描述：人牙本质基质蛋白1(DMP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DMP1含量。人牙本质基质蛋白1（DMP1）是一种在牙齿发育和生物矿化过程中起关键作用的蛋白质。它在牙本质形成、矿化及牙本质修复等过程中发挥着重要作用，同时也是常染色体隐性低血磷性佝偻病和非矿化组织疾病发病机制研究的重要靶点。DMP1通过调节羟磷灰石的成核作用启动矿化过程，并参与细胞间信号传递。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域中对牙齿发育机制、骨组织再生调控或生物材料矿化效应的研究，为探索矿化相关疾病的分子机制或组织工程研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DMP1 ELISA Kit; Dentin matrix acidic phosphoprotein 1 ELISA Kit; DMP-1 ELISA Kit; Dentin matrix protein 1 ELISA Kit
缩写：DMP1
Uniprot No.：Q13316
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-91 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-105 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 98-108 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-98 | |||||
回收率：
| The recovery of human DMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-95 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.6882.7222.7052.586 102.3992.2922.3462.227 51.8941.8861.8901.771 2.51.0741.0611.0680.949 1.250.7780.7880.7830.664 0.6250.3990.3860.3930.274 0.3120.2630.2480.2560.137 00.1180.1200.119 | ||||||
货期：3-5 working days
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