首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血管抑素/血管稳定蛋白(ANG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的ANG含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外研究ANG在心血管疾病、肿瘤血管生成、伤口愈合等领域的表达调控机制，或用于评估药物干预对血管生成相关通路的影响。在肿瘤微环境、缺血性疾病及炎症相关研究中具有重要价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：ANG
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human angiostatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:40 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:80 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:160 | Average % | 96 | ||||
| Range % | 92-100 | |||||
回收率：
| The recovery of Human angiostatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.5562.6982.6272.535502.2172.1162.1672.075251.4491.5461.4981.40612.50.7950.7830.7890.6976.250.3980.3850.3920.3003.120.2770.2820.2800.1881.560.1590.1640.1620.07000.0910.0920.092 | ||||||
货期：3-5 working days
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