首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脑啡肽(ENK)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的ENK含量。人脑啡肽（ENK）是一种内源性阿片肽，具有镇痛、调节情绪和行为等功能。其研究机制涉及与脑内阿片受体结合，通过抑制疼痛信号传递和调节神经递质来发挥作用。靶点背景研究显示，ENK主要作用于μ、δ、κ和痛敏肽受体，其生物学功能广泛，与多种生理和病理过程相关。试剂盒检测范围为0.05 ng/mL-10 ng/mL，适用于科研领域对神经递质调控机制的探索；可应用于神经生物学、药理学研究中ENK动态变化的检测，或用于评估药物干预、行为学模型对脑啡肽系统的影响，为探索神经系统功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ENK
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.05 ng/mL-10 ng/mL
灵敏度：0.025 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 92-99 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-100 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-99 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 82-102 | |||||
回收率：
| The recovery of human ENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-102 | ||||
| EDTA plasma (n=4) | 97 | 93-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 100.1970.2010.199 2.50.3660.3520.359 0.80.6130.6280.621 0.21.0141.0061.010 0.051.4481.4251.437 02.4392.4492.444 | ||||||
货期：3-5 working days
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