首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人硫氧还蛋白还原酶(TrxR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的TXNRD1含量。TXNRD1 是重要的氧化还原调节酶，在维持细胞内氧化还原平衡等方面有重要作用。其异常表达与肿瘤等多种疾病发生发展相关。研究机制主要聚焦于它参与的氧化还原信号通路，通过调控相关分子活性来影响细胞生理病理过程，是潜在治疗靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外研究TrxR在氧化应激相关疾病模型中的表达变化，或评估药物干预对TrxR活性调控的影响，为分子机制研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cytoplasmic ELISA Kit; Gene associated with retinoic and IFN-induced mortality 12 protein ELISA Kit; Gene associated with retinoic and interferon-induced mortality 12 protein ELISA Kit; Gene associated with retinoid IFN induced mortality 12 protein ELISA Kit; GRIM 12 ELISA Kit; GRIM-12 ELISA Kit; GRIM12 ELISA Kit; KDRF ELISA Kit; KM 102 derived reductase like factor ELISA Kit; KM-102-derived reductase-like factor ELISA Kit; MGC9145 ELISA Kit; Oxidoreductase ELISA Kit; Thioredoxin reductase 1 ELISA Kit; Thioredoxin reductase 1 cytoplasmic ELISA Kit; Thioredoxin reductase GRIM 12 ELISA Kit; Thioredoxin reductase TR1 ELISA Kit; TR 1 ELISA Kit; TR ELISA Kit; TR1 ELISA Kit; TRXR 1 ELISA Kit; TRXR1 ELISA Kit; TRXR1_HUMAN ELISA Kit; TXNR ELISA Kit; TXNRD 1 ELISA Kit; Txnrd1 ELISA Kit
缩写：TXNRD1
Uniprot No.：Q16881
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TrxR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 99 | |||||
| Range % | 87-104 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 94-105 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 85-99 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 84-105 | ||||||
回收率：
| The recovery of human TrxR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 83-94 | |||||
| EDTA plasma (n=4) | 102 | 91-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected201.9941.9431.9691.787101.5551.5011.5281.34651.0911.0621.0770.8952.50.7760.7840.7800.5981.250.4840.4760.4800.2980.6250.3590.3470.3530.1710.3120.2700.2630.2670.08500.1850.1790.182 | |||||||
货期：3-5 working days
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