首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人赖氨酰氧化酶酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LOX含量。LOX即赖氨酰氧化酶，是一种铜依赖的胺氧化酶。其与多种生理病理过程相关，如促进细胞外基质交联、维持组织张力稳定。研究显示它在肿瘤微环境调控中起作用，可影响肿瘤细胞增殖、转移等，是潜在的疾病治疗靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于精准分析低丰度样本，适用于基础研究中LOX蛋白表达水平的动态监测，例如探索纤维化疾病模型中LOX的调控机制、评估肿瘤微环境中基质重塑的分子特征，或研究心血管疾病中细胞外基质代谢异常等方向，为体外实验提供可靠的定量数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：lox ELISA Kit; LYOX ELISA Kit; LYOX_HUMAN ELISA Kit; Lysyl oxidase ELISA Kit; MGC105112 ELISA Kit; Protein lysine 6 oxidase ELISA Kit; Protein-lysine 6-oxidase ELISA Kit
缩写：LOX
Uniprot No.：P28300
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LOX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 86-95 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human LOX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-107 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.6332.5522.5932.495201.8321.7761.8041.706101.1041.1301.1171.01950.5390.5620.5510.4532.50.3240.3350.3300.2321.250.2350.2190.2270.1290.6250.1850.1790.1820.08400.1020.0940.098 | ||||||
货期：3-5 working days
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