首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人饥饿激素(GHRL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的GHRL含量。GHRL是胃饥饿素的英文缩写，它由胃分泌，能调节食欲、能量代谢和生长激素释放等。目前研究主要聚焦于其与肥胖、糖尿病等代谢疾病的关联机制，探究通过调控GHRL及其受体来干预疾病，有望为这些代谢疾病治疗提供新策略。试剂盒检测范围为0.625 pg/mL-40 pg/mL，本试剂盒适用于体外科研场景，例如探究能量代谢调控机制、评估药物或营养干预对GHRL表达的影响，以及疾病模型中激素水平动态变化的监测。血清和血浆样本可用于动物或人类临床前研究，细胞培养上清适用于体外细胞分泌功能分析，而组织匀浆则有助于探究GHRL在特定器官中的表达特征。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Appetite regulating hormone ELISA Kit; Ghrelin 27 ELISA Kit; Ghrelin 28 ELISA Kit; Ghrelin and obestatin prepropeptide ELISA Kit; Ghrelin ELISA Kit; Ghrelin/obestatin prepropeptide ELISA Kit; ghrl ELISA Kit; GHRL_HUMAN ELISA Kit; Growth hormone releasing peptide ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; M46 protein ELISA Kit; Motilin related peptide ELISA Kit; Motilin-related peptide ELISA Kit; MTLRP ELISA Kit; Obestatin ELISA Kit; Protein M46 ELISA Kit
缩写：GHRL
Uniprot No.：Q9UBU3
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 pg/mL-40 pg/mL
灵敏度：0.156 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 99 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 101 | ||||
| Range % | 92-110 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 94 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected402.9212.9032.9122.826202.4052.3482.3832.297101.6631.6431.6531.56751.0530.9891.0210.9352.50.5750.6030.5890.5031.250.3220.3190.3210.2350.6250.2140.2290.2220.13600.0890.0830.086 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human GHRL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled GHRL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
×
